- Akron Akron Children's Hospital
- Atlanta Aflac Center for Cancer and Blood Disorders Service at Children's Healthcare of Atlanta
- Baltimore University of Maryland Medical Center, Hospital for Children
- Baltimore Johns Hopkins Children's Center
- Boston The Floating Hospital for Children - Tufts New England Medical Center
- Boston Children's Hopsital Boston
- Buffalo Women & Children's Hospital of Buffalo
- Charlotte Presbyterian Healthcare
- Cleveland Cleveland Clinic Children's Hospital
- Cleveland Rainbow Babies & Children's Hospital
- Charlotte Presbyterian Hemby Children's Hospital
- Chicago Comer Children's Hospital
- Columbus Nationwide Children's Hospital
- Denver The Children's Hospital, Colorado
- Houston MD Anderson Cancer Center
- Houston Texas Children's Hospital
- Indianapolis Riley Hospital for Children
- Indianapolis St. Vincent Indianapolis Hospital
- Kansas City - Opening in 2008
- Las Vegas Sunrise Children's Hospital
- Los Angeles - Opening in 2008
- Madison American Family Children's Hospital - UW
- Milwaukee - Opening in 2008
- Minneapolis Children's Hospitals and Clinics of Minnesota
- New Brunswick - Opening in 2008
- New York Stephen D. Hassenfeld Center for Cancer and Blood Disorders at NYU Medical Center
- Norfolk Children's Hospital of the King's Daughters
- North Carolina Triangle North Carolina Children's Hospital - UNC
- Northern Virginia Inova Fairfax Hospital for Children
- Orlando Arnold Palmer Hospital for Children
- Philadelphia Children's Hospital of Philadelphia
- Pittsburgh Children's Hospital of Pittsburgh
- Phoenix Phoenix Children's Hospital
- San Francisco UCSF Children's Hospital
- Tampa Bay All Children's Hospital
- Valerie Fund Centers - Opening in 2008
